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CoraLite®594-conjugated HSP47 Monoclonal antibody

HSP47 Monoclonal Antibody for IF, FC (intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A8F6

Cat no : CL594-67863

Synonyms

47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated HSP47 Monoclonal antibody (CL594-67863)

    IF Staining of HeLa using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated HSP47 Monoclonal antibody (CL594-67863)

    IF Staining of HepG2 using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated HSP47 Monoclonal antibody (CL594-67863)

    FC experiment of HepG2 using CL594-67863

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HSP47 (CL594-67863, Clone:2A8F6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67863 targets HSP47 in IF, FC (intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP47 fusion protein Ag30999
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC014623
Gene Symbol SERPINH1
Gene ID (NCBI) 871
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CL594 HSP47 antibody CL594-67863Download protocol
Standard Protocols
Click here to view our Standard Protocols