HSN2 Polyclonal antibody

HSN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24055-1-AP

Synonyms

HSAN2, HSN2

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Validation Data Gallery

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  WB analysis of mouse brain using 24055-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24055-1-AP (HSN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24055-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24055-1-AP (HSN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 24055-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 24055-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells
• Positive IHC detected in: human kidney tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24055-1-AP is HSN2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSN2 fusion protein Ag21184
• Full Name: hereditary sensory neuropathy, type II
• Calculated Molecular Weight: 2634 aa, 280 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC130469
• Gene Symbol: HSN2
• Gene ID (NCBI): 378465
• RRID: AB_2879417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSN2, also named as WNK1, KDP, KIAA0344, PRKWNK1, P65 and hWNK1, is a serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. It acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. WNK1 may also play a role in actin cytoskeletal reorganization. This antibody detects the 65 kDa protein (Erythrocyte 65 kDa protein, P65).