HSD17B3 Polyclonal antibody

HSD17B3 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13415-1-AP

Synonyms

17 beta HSD 3, EDH17B3, HSD17B3, SDR12C2

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Validation Data Gallery

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  IHC staining of human testis using 13415-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13415-1-AP (HSD17B3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13415-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13415-1-AP (HSD17B3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

13415-1-AP targets HSD17B3 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B3 fusion protein Ag4081
• Full Name: hydroxysteroid (17-beta) dehydrogenase 3
• Calculated Molecular Weight: 310 aa, 35 kDa
• GenBank Accession Number: BC034281
• Gene Symbol: HSD17B3
• Gene ID (NCBI): 3293
• RRID: AB_2877946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Male germ cells support long-term propagation of Zika virus.; Authors - Christopher L Robinson
human	IHC	J Steroid Biochem Mol Biol; Features of the fetal gonad in androgen synthesis in the postpubertal testis are preserved in complete androgen insensitivity syndrome due to a novel genetic splice site donor variant in androgen receptor gene intron 1.; Authors - Katarzyna Jarzabek
rat	IF	Syst Biol Reprod Med; Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering; Authors - Vitória Frias Batista
human	IHC	J Endocr Soc; Precocious Puberty in a Boy With Bilateral Leydig Cell Tumors due to a Somatic Gain-of-Function LHCGR Variant; Authors - Chelsi Flippo
human	IHC	Eur J Endocrinol; Persistence of foetal testicular features in patients with defective androgen signalling; Authors - Mostafa Al-Sharkawi
mouse	IHC	Biochem Biophys Res Commun; Prepubertal exposure to copper oxide nanoparticles induces Leydig cell injury with steroidogenesis disorders in mouse testes; Authors - Xiangqin Zheng