VILIP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

VILIP3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10989-1-AP

Synonyms

BDR1, Calcium binding protein BDR 1, hippocalcin like 1, Hippocalcin like protein 1, HLP2, HPCA, HPCAL1, VILIP 3, VILIP-3, Visinin like protein 3

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Validation Data Gallery

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  WB analysis using 10989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10989-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10989-1-AP

  IP result of anti-VILIP3 (IP:10989-1-AP, 4ug; Detection:10989-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human kidney using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse cerebellum using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10989-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VILIP3 antibody (10989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, mouse brain tissue, rat brain tissue, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10989-1-AP targets VILIP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VILIP3 fusion protein Ag1449
• Full Name: hippocalcin-like 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009846
• Gene Symbol: HPCAL1
• Gene ID (NCBI): 3241
• RRID: AB_2120220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VILIP3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system.

Publications

Species	Application	Title
human	WB	Autophagy; Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis; Authors - Xin Chen ; KD Validated
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB,IHC	Hepatology; Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization via activating ERK1/2-MAPK pathway.; Authors - Yonglong Zhang ; KD Validated
human	WB,IHC	Cancer Med; Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments.; Authors - Mingjian Ma
human,mouse	WB	Theranostics; Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling; Authors - Tao Chen
human	IHC	Signal Transduct Target Ther; Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression; Authors - Xiang-Peng Tan