HOXD13 Polyclonal antibody

HOXD13 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18736-1-AP

Synonyms

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

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Validation Data Gallery

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  WB analysis of A549 using 18736-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18736-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18736-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 18736-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 18736-1-AP

  IP result of anti-HOXD13 (IP:18736-1-AP, 4ug; Detection:18736-1-AP 1:500) with A549 cells lysate 1600ug.

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  IHC staining of human brain using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, rat brain tissue, Jurkat cells, mouse lung tissue, mouse brain tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human brain tissue, human hepatocirrhosis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18736-1-AP targets HOXD13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: homeobox D13
• Calculated Molecular Weight: 343 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC148863
• Gene Symbol: HOXD13
• Gene ID (NCBI): 3239
• RRID: AB_2119926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues.

Publications

Species	Application	Title
		Mol Pharm; Sialic Acid-Functionalized PEG-PLGA Microspheres Loading Mitochondrial Targeting Modified Curcumin for Acute Lung Injury Therapy.; Authors - Fei-Yang Jin
mouse	WB	Cell Death Dis; A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis; Authors - Lishan Zhang