HOXC10 Polyclonal antibody

HOXC10 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20632-1-AP

Synonyms

homeobox C10, Homeobox protein Hox 3I, Homeobox protein Hox C10, HOX3I, HOXC10

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Validation Data Gallery

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  WB analysis of HeLa using 20632-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20632-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 20632-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20632-1-AP (HOXC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20632-1-AP is HOXC10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXC10 fusion protein Ag14620
• Full Name: homeobox C10
• Calculated Molecular Weight: 342 aa, 38 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC001293
• Gene Symbol: HOXC10
• Gene ID (NCBI): 3226
• RRID: AB_10697666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)