HOXB13 Polyclonal antibody

HOXB13 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26384-1-AP

Synonyms

homeobox B13, Homeobox protein Hox B13, HOXB13, PSGD

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Validation Data Gallery

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  WB analysis using 26384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26384-1-AP (HOXB13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 26384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26384-1-AP (HOXB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26384-1-AP (HOXB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 26384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HOXB13 antibody (26384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26384-1-AP targets HOXB13 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXB13 fusion protein Ag24757
• Full Name: homeobox B13
• Calculated Molecular Weight: 284 aa, 31 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC007092
• Gene Symbol: HOXB13
• Gene ID (NCBI): 10481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The homeobox (HOX) is a specific and widely distributed DNA sequence characterizing genes that are responsive to HOX transcription factors. Homeobox B13 (HOXB13) is one of the numerous DNA-binding transcription factors of the HOX gene family that has been implicated in the development of normal prostate and cancer (PMID: 25825985). In addition, high-level expression of HOXB13 is closely associated with tumor angiogenesis and poor prognosis of hepatocellular carcinoma(PMID: 25031711).