CoraLite® Plus 488-conjugated HOXA7 Monoclonal antibody

HOXA7 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A7H6

Cat no : CL488-67112

Synonyms

ANTP, homeobox A7, Homeobox protein Hox 1.1, Homeobox protein Hox 1A, Homeobox protein Hox A7, HOX1, HOX1.1, HOX1A, HOXA7

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67112

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HOXA7 antibody (CL488-67112, Clone: 2A7H6 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-67112

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HOXA7 antibody (CL488-67112, Clone: 2A7H6 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67112 targets HOXA7 in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HOXA7 fusion protein Ag19025
• Full Name: homeobox A7
• Calculated Molecular Weight: 230 aa, 25 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC148692
• Gene Symbol: HOXA7
• Gene ID (NCBI): 3204
• RRID: AB_2919422
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOX genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene Hoxa-1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor, which has been shown to play important roles in cell differentiation and embryogenesis. Hoxa-1 is also expressed in cancers, such as mammary tumors, though it is not expressed in normal gland or in precancerous mammary tissues. At embryonic stages, Hoxa-2 is expressed in the mesenchyme and epithelial cells of palate, however its expression is restricted to the tips of the growing palatal shelves. Hoxa-2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Hoxa-7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. Hoxa-7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta.