HOXA7 Monoclonal antibody
HOXA7 Monoclonal Antibody for WB, IF, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2A7H6
Cat no : 67112-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells, HepG2 cells, SMMC-7721 cells, HT-29 cells, HL-60 cells, K-562 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
67112-1-Ig targets HOXA7 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | HOXA7 fusion protein Ag19025 |
Full Name | homeobox A7 |
Calculated Molecular Weight | 230 aa, 25 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC148692 |
Gene Symbol | HOXA7 |
Gene ID (NCBI) | 3204 |
RRID | AB_2882416 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HOX genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene Hoxa-1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor, which has been shown to play important roles in cell differentiation and embryogenesis. Hoxa-1 is also expressed in cancers, such as mammary tumors, though it is not expressed in normal gland or in precancerous mammary tissues. At embryonic stages, Hoxa-2 is expressed in the mesenchyme and epithelial cells of palate, however its expression is restricted to the tips of the growing palatal shelves. Hoxa-2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Hoxa-7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. Hoxa-7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HOXA7 antibody 67112-1-Ig | Download protocol |
IF protocol for HOXA7 antibody 67112-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Reprod Sci CircPAPPA Regulates the Proliferation, Migration, Invasion, Apoptosis, and Cell Cycle of Trophoblast Cells Through the miR-3127-5p/HOXA7 Axis. | ||
J Orthop Surg Res MiR-920 promotes osteogenic differentiation of human bone mesenchymal stem cells by targeting HOXA7. | ||
Cancer Sci Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment
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