HNRNPL Recombinant antibody

HNRNPL Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3J22

Cat no : 82259-1-RR

Synonyms

FLJ35509, hnRNP L, HNRNPL, HNRPL, P/OKcl.14

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Validation Data Gallery

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  WB analysis using 82259-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82259-1-RR (HNRNPL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, BxPC-3 cells, C6 cells, HSC-T6 cells
• Positive IHC detected in: human stomach cancer tissue, human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:19600
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82259-1-RR targets HNRNPL in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HNRNPL fusion protein Ag13211
• Full Name: heterogeneous nuclear ribonucleoprotein L
• Calculated Molecular Weight: 572 aa, 62 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC069184
• Gene Symbol: HNRNPL
• Gene ID (NCBI): 3191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.