HNRNPL Polyclonal antibody

• Featured Product
• KD/KO Validated

HNRNPL Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 18354-1-AP

Synonyms

hnRNP L, HNRNPL, HNRPL, P/OKcl.14

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Validation Data Gallery

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  WB analysis using 18354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18354-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 18354-1-AP

  IP result of anti-HNRNPL (IP:18354-1-AP, 4ug; Detection:18354-1-AP 1:4000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18354-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPL antibody (18354-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse liver tissue, PC-3 cells, RAW 264.7 cells
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: human stomach cancer tissue, human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

18354-1-AP targets HNRNPL in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, hamster, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPL fusion protein Ag13211
• Full Name: heterogeneous nuclear ribonucleoprotein L
• Calculated Molecular Weight: 572 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC069184
• Gene Symbol: HNRNPL
• Gene ID (NCBI): 3191
• RRID: AB_2878537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.

Publications

Species	Application	Title
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	ChIP	RNA Biol; CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation.; Authors - Yi Ran
human	WB	J Proteome Res; C1QBP Negatively Regulates the Activation of Oncoprotein YBX1 in the Renal Cell Carcinoma as Revealed by Interactomics Analysis.; Authors - Yong Wang
pig,bovine,hamster	WB,IF	J Virol; Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-and-Mouth Disease Virus Replication through Inhibition of Viral RNA Synthesis by Interacting with the Internal Ribosome Entry Site in the 5' Untranslated Region.; Authors - Chao Sun ; KO Validated
human	WB	Methods; Fast and unbiased purification of RNA-protein complexes after UV cross-linking.; Authors - Erika C Urdaneta
human	IHC	DNA Cell Biol; HNRNPL Is Identified and Validated as a Prognostic Biomarker Associated with Microsatellite Instability in Human Gastric Cancer.; Authors - Xiongjie Yu