HNMT Polyclonal antibody

HNMT Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11874-1-AP

Synonyms

histamine N methyltransferase, HMT, HNMT, HNMT S1, HNMT S2

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Validation Data Gallery

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  WB analysis using 11874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11874-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11874-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11874-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11874-1-AP (HNMT antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11874-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11874-1-AP (HNMT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-29 cells, human liver tissue, mouse liver tissue, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11874-1-AP targets HNMT in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNMT fusion protein Ag2462
• Full Name: histamine N-methyltransferase
• Calculated Molecular Weight: 292 aa, 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC020677
• Gene Symbol: HNMT
• Gene ID (NCBI): 3176
• RRID: AB_2117048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.

Publications

Species	Application	Title
human	IHC	Glia; Molecular mechanism of histamine clearance by primary human astrocytes.; Authors - Yoshikawa Takeo T
human	WB	Cell Stem Cell; Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling; Authors - Jiayi Chen