HMGN4 Polyclonal antibody

HMGN4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11686-1-AP

Synonyms

HMG17L3, HMGN4, NHC

---

Validation Data Gallery

• 

  WB analysis of HeLa using 11686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11686-1-AP (HMGN4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 11686-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11686-1-AP (HMGN4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11686-1-AP targets HMGN4 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGN4 fusion protein Ag2298
• Full Name: high mobility group nucleosomal binding domain 4
• Calculated Molecular Weight: 90 aa, 10 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001282
• Gene Symbol: HMGN4
• Gene ID (NCBI): 10473
• RRID: AB_2118489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGN4, also named as High mobility group nucleosome-binding domain-containing protein 4, is a 90 amino acid protein, which belongs to the HMGN family. HMGN4 as a DNA-binding protein localizes in the nucleus. The calculated molecular weight of HMGN4 is 10 kDa, but the modified HMGN4 protein is about 15 kDa.