HMGA1 Polyclonal antibody

HMGA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29895-1-AP

Synonyms

high mobility group AT hook 1, High mobility group protein A1, High mobility group protein R, HMG I(Y), HMG R, HMGA1, HMGA1A, HMGIY

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Validation Data Gallery

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  WB analysis using 29895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29895-1-AP (HMGA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, MKN-45 cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29895-1-AP targets HMGA1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGA1 fusion protein Ag31866
• Full Name: high mobility group AT-hook 1
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC071864
• Gene Symbol: HMGA1
• Gene ID (NCBI): 3159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).

Publications

Species	Application	Title
human	WB	Pathol Oncol Res; Long non-coding RNA MYU promotes ovarian cancer cell proliferation by sponging miR-6827-5p and upregulating HMGA1; Authors - Shaoyu Wang