HMBOX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HMBOX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16123-1-AP

Synonyms

HMBOX1, HNF1LA, homeobox containing 1, Homeobox containing protein 1, PBHNF

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Validation Data Gallery

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16123-1-AP

  IP result of anti-HMBOX1 (IP:16123-1-AP, 4ug; Detection:16123-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

16123-1-AP targets HMBOX1 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMBOX1 fusion protein Ag9081
• Full Name: homeobox containing 1
• Calculated Molecular Weight: 420 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC009259
• Gene Symbol: HMBOX1
• Gene ID (NCBI): 79618
• RRID: AB_10794445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	ChIP	Mol Ther; Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.; Authors - Chenfei Zhou ; KD Validated
human,rat	WB	Front Cardiovasc Med; Exosome-Derived From Sepsis Patients' Blood Promoted Pyroptosis of Cardiomyocytes by Regulating miR-885-5p/HMBOX1.; Authors - Guo-Wei Tu
		Oncol Rep; Knockdown of homeobox containing 1 increases the radiosensitivity of cervical cancer cells through telomere shortening.; Authors - Shuliang Zhou
human	WB	Exp Ther Med; HMBOX1 attenuates LPS-induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF-κB signaling pathway.; Authors - Minyuan Nie
human	WB,IHC	Cell Death Dis; WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m6A-dependent manner.; Authors - Shijie Chen ; KD Validated
human	WB	J Cell Mol Med; 5,2'-dibromo-2,4',5'-trihydroxydiphenylmethanone attenuates LPS-induced inflammation and ROS production in EA.hy926 cells via HMBOX1 induction.; Authors - Hong-Xia Yuan