HIST1H1B Polyclonal antibody

HIST1H1B Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18093-1-AP

Synonyms

H1, H1.5, H1F5, HIST1H1B, histone cluster 1, H1b, Histone H1.5, Histone H1a

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Validation Data Gallery

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  WB analysis using 18093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (HIST1H1B antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 18093-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HIST1H1B antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, LnCaP cells
• Positive IHC detected in: mouse brain tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:30000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18093-1-AP targets HIST1H1B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIST1H1B fusion protein Ag12818
• Full Name: histone cluster 1, H1b
• Calculated Molecular Weight: 226 aa, 23 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC101581
• Gene Symbol: HIST1H1B
• Gene ID (NCBI): 3009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone Cluster 1 H1 Family Member B (HIST1H1B) is a member of the Histone H1/H5 protein family. HIST1H1B gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. HIST1H1B expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).