HIPK3 Polyclonal antibody

HIPK3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25107-1-AP

Synonyms

ANPK, DYRK6, FIST, FIST3, HIPK3, Homolog of protein kinase YAK1, PKY, YAK1



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25107-1-AP targets HIPK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIPK3 fusion protein Ag18897
Full Name homeodomain interacting protein kinase 3
Calculated Molecular Weight 1215 aa, 134 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC156873
Gene Symbol HIPK3
Gene ID (NCBI) 10114
RRIDAB_2879899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HIPK3 antibody 25107-1-APDownload protocol
IHC protocol for HIPK3 antibody 25107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Identification of HIPK3 as a potential biomarker and an inhibitor of clear cell renal cell carcinoma.

Authors - Wen Xiao
humanWB,IHC

Sci Rep

Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3

Authors - Aye Myat Mon