HELLS Polyclonal antibody

HELLS Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (2)

Cat no : 11955-1-AP

Synonyms

helicase, lymphoid, HELLS, LSH, Lymphoid specific helicase, Nbla10143, PASG, SMARCA6

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Validation Data Gallery

• 

  WB analysis of K-562 using 11955-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11955-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11955-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 11955-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11955-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11955-1-AP

  IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IF Staining of HepG2 using 11955-1-AP

  Immunofluorescent analysis of HepG2 cells, using HELLS antibody 11955-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: K-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells
• Positive IP detected in: mouse testis tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Product Information

11955-1-AP targets HELLS in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HELLS fusion protein Ag2558
• Full Name: helicase, lymphoid-specific
• Calculated Molecular Weight: 348 aa, 40 kDa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC031004
• Gene Symbol: HELLS
• Gene ID (NCBI): 3070
• RRID: AB_2117529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

Publications

Species	Application	Title
	WB	Nat Commun; Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells; Authors - Clara Cousu
mouse	WB	Sci Adv; CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses; Authors - Maja Vukic
mouse	WB, IF	Genome Biol; Lsh regulates LTR retrotransposon repression independently of Dnmt3b function.; Authors - Donncha S Dunican
human	WB	mSystems; Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes; Authors - Joseph M. Dybas
		Hum Mol Genet; Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.; Authors - Haoyu Wu
human	WB	Cancer Sci; Lymphoid-specific helicase promotes the growth and invasion of hepatocellular carcinoma by transcriptional regulation of centromere protein F expression.; Authors - Xuan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Diana (Verified Customer) (08-18-2022)

In IF the antibody binds specific in the nucleus and has low background, thats great! In WB I have a band as aspected around 100kd but I also have some other smaller unspecific bands. Here I will work on the troubleshooting. Thanks for the corresponding protocolls with buffers and incubation time.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500 (WB), 1:25 (IF)
• Cell Tissue Type: mouse testis, GC1, NIH3T3

FH

Michelle (Verified Customer) (10-24-2019)

Great antibody for HELLS. Customer support was really helpful when I was troubleshooting how to clean up my western blot. Changed the path of my project since multiple bands for different isoforms of the protein appeared in the cell lines I was invesitgating.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Jurkats, HAP1, PBMC