HEBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HEBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16842-1-AP

Synonyms

HBP, HEBP, HEBP1, heme binding protein 1, p22HBP

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Validation Data Gallery

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  WB analysis using 16842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16842-1-AP (HEBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16842-1-AP

  WB result of HEBP1 antibody (16842-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

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  IHC staining of human liver cancer using 16842-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16842-1-AP (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16842-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16842-1-AP (HEBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: L02 cells, A549 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16842-1-AP targets HEBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEBP1 fusion protein Ag10538
• Full Name: heme binding protein 1
• Calculated Molecular Weight: 189 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC016277
• Gene Symbol: HEBP1
• Gene ID (NCBI): 50865
• RRID: AB_2117378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEBP1 (heme binding protein 1) is an intracellular tetrapyrrole-binding protein. Catalog#16842-1-AP is a rabbit polyclonal antibody raised against the full-length of human HEBP1. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein.