HDLBP Polyclonal antibody

• Featured Product
• KD/KO Validated

HDLBP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15406-1-AP

Synonyms

HBP, HDL binding protein, HDLBP, PRO2900, VGL, Vigilin

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Validation Data Gallery

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  WB analysis using 15406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15406-1-AP (HDLBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15406-1-AP

  IP result of anti-HDLBP (IP:15406-1-AP, 4ug; Detection:15406-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of mouse stomach using 15406-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15406-1-AP (HDLBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 15406-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15406-1-AP (HDLBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 15406-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HDLBP antibody 15406-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

15406-1-AP targets HDLBP in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDLBP fusion protein Ag7666
• Full Name: high density lipoprotein binding protein
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141 kDa
• GenBank Accession Number: BC001179
• Gene Symbol: HDLBP
• Gene ID (NCBI): 3069
• RRID: AB_2117367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Exp Cell Res; A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers.; Authors - Wei Zhou ; KD Validated
human	WB	J Med Virol; Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis.; Authors - Zhao Chao C
human	WB,IHC,IF,CoIP	Cell Mol Gastroenterol Hepatol; HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation; Authors - Jingsheng Yuan