HDDC2 Monoclonal antibody

HDDC2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E1B2

Cat no : 68391-1-Ig

Synonyms

C6orf74, CGI 130, dJ167O5.2, HD domain containing 2, HD domain containing protein 2, HDDC2, NS5ATP2

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Validation Data Gallery

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  WB analysis using 68391-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68391-1-Ig (HDDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Saos-2 using 68391-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using HDDC2 antibody (68391-1-Ig, Clone: 2E1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells
• Positive IF detected in: Saos-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68391-1-Ig targets HDDC2 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDDC2 fusion protein Ag33258
• Full Name: HD domain containing 2
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC003357
• Gene Symbol: HDDC2
• Gene ID (NCBI): 51020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.