HCRTR1 Polyclonal antibody

HCRTR1 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18370-1-AP

Synonyms

HCRTR1, hypocretin (orexin) receptor 1, Hypocretin receptor type 1, Orexin receptor 1, Orexin receptor type 1, Ox 1 R, Ox1 R, OX1R

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Validation Data Gallery

• 

  WB analysis of mouse brain using 18370-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18370-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18370-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 18370-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 18370-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Orexin receptor 1 antibody (18370-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain tissue, Jurkat cells, mouse pancreas tissue, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

18370-1-AP targets HCRTR1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HCRTR1 fusion protein Ag12269
• Full Name: hypocretin (orexin) receptor 1
• Calculated Molecular Weight: 425 aa, 48 kDa
• Observed Molecular Weight: 48-54 kDa
• GenBank Accession Number: BC074796
• Gene Symbol: HCRTR1
• Gene ID (NCBI): 3061
• RRID: AB_10858633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).

Publications

Species	Application	Title
mouse	WB,IHC	Front Pharmacol; The Ciji-Hua'ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma.; Authors - Shengyan Xi
mouse	WB	Brain Res Bull; Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism.; Authors - Pedro P M Scariot
mouse	WB	Drug Des Devel Ther; Orexin-A Reverse Bone Mass Loss Induced by Chronic Intermittent Hypoxia Through OX1R-Nrf2/HIF-1α Pathway.; Authors - Hong Gu
rat	WB	Evid Based Complement Alternat Med; Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A.; Authors - Liang-Hui Zhan
human	WB	Biochim Biophys Acta Mol Basis Dis; Orexin-A/OX1R is involved in regulation of autophagy to promote cortisol secretion in adrenocortical cell; Authors - Xin Guo
mouse	IF	Nat Biotechnol; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells; Authors - Yunlong Tao