HCFC1 Polyclonal antibody

HCFC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19358-1-AP

Synonyms

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

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Validation Data Gallery

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  WB analysis of HEK-293 using 19358-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19358-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

19358-1-AP targets HCFC1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HCFC1 fusion protein Ag6798
• Full Name: host cell factor C1 (VP16-accessory protein)
• Calculated Molecular Weight: 209 kDa
• Observed Molecular Weight: 230 kDa, 300 kDa
• GenBank Accession Number: BC063435
• Gene Symbol: HCFC1
• Gene ID (NCBI): 3054
• RRID: AB_10860105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Host cell factor 1 (HCFC1), also known as HCF-1, is a transcriptional co-regulator with a proteolytic cleavage at the PRO repeats that could be cleaved by O-GlcNAc transferase (OGT) to produce a heterodimeric complex of HCFC1N and HCFC1C subunits (PMID: 23539139). HCFC1 was first identified in herpes simplex virus transcription while it lacks a DNA binding domain. HCFC1 is not a traditional transcription factor, it interacts with multiple protein including transcription factors to regulate many biological processes, such as cell proliferation, migration, cell death and mitochondrial biogenesis (PMID: 25281006). Mutation in HCFC1 was related to an X-linked recessive disease ---cblX with a complex clinical manifestation involved in error of metabolism,  neurocognitive impairment and  craniofacial abnormalities (PMID: 24011988; PMID: 25281006).

Publications

Species	Application	Title
human,rat	IF	Microbiol Immunol; Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation-dependent.; Authors - Erina Minato