HAT1 Monoclonal antibody

• Featured Product
• KD/KO Validated

HAT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11F6

Cat no : 67971-1-Ig

Synonyms

HAT1, histone acetyltransferase 1, KAT1

---

Validation Data Gallery

• 

  WB analysis using 67971-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67971-1-Ig (HAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HEK-293 using 67971-1-Ig

  WB result of HAT1 antibody (67971-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

---

Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67971-1-Ig targets HAT1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HAT1 fusion protein Ag1952
• Full Name: histone acetyltransferase 1
• Calculated Molecular Weight: 419 aa, 50 kDa
• Observed Molecular Weight: 45-46 kDa
• GenBank Accession Number: BC018682
• Gene Symbol: HAT1
• Gene ID (NCBI): 8520
• RRID: AB_2918721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.