GTF2A1 Polyclonal antibody

GTF2A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21381-1-AP

Synonyms

GTF2A1, TF2A1, TFIIA, TFIIA 42, TFIIA p19 subunit, TFIIAL



Tested Applications

Positive WB detected inrat testis tissue, BGC-823 cells, SKOV-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21381-1-AP is GTF2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2A1 fusion protein Ag15994
Full Name general transcription factor IIA, 1, 19/37kDa
Calculated Molecular Weight 276 aa, 42 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC107156
Gene Symbol GTF2A1
Gene ID (NCBI) 2957
RRIDAB_2878853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GTF2A1 antibody 21381-1-APDownload protocol
IHC protocol for GTF2A1 antibody 21381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols