GSTM5 Monoclonal antibody

GSTM5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2E5C2

Cat no : 68210-1-Ig

Synonyms

glutathione S transferase mu 5, GST class mu 5, GSTM5, GSTM5 5, GTM5

---

Validation Data Gallery

• 

  WB analysis using 68210-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68210-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 68210-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: rabbit brain tissue, pig liver tissue, rabbit liver tissue, rat brain tissue, mouse brain tissue, mouse liver tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68210-1-Ig targets GSTM5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSTM5 fusion protein Ag6054
• Full Name: glutathione S-transferase mu 5
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC058881
• Gene Symbol: GSTM5
• Gene ID (NCBI): 2949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM5, Glutathione S-transferases, belongs to eukaryotic and prokaryotic phase II metabolic isozymes family. It can catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for detoxification. GSTM5 undergo epigenetic repression in AMD (age-related macular degeneration) RPE/choroid, which may increase susceptibility to oxidative stress in AMD retinas (PMID: 22410570). GSTM5 was found in brain, lung and testis (PMID: 9230131).

Publications

Species	Application	Title
human	WB	Lasers Med Sci; Revealing the mechanism of 755-nm long-pulsed alexandrite laser in inhibiting infantile hemangioma endothelial cells through transcriptome sequencing; Authors - Chen Ke