eRF3a/GSPT1 Monoclonal antibody

eRF3a/GSPT1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A3H6

Cat no : 68217-1-Ig

Synonyms

551G9.2, eRF3a, ETF3A, FLJ38048, FLJ39067, G1 to S phase transition 1, GSPT1, GST1

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Validation Data Gallery

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  WB analysis using 68217-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68217-1-Ig (eRF3a/GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of COLO 320 using 68217-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 68217-1-Ig (GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68217-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68217-1-Ig (GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 68217-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using eRF3a/GSPT1 antibody (68217-1-Ig, Clone: 2A3H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 68217-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eRF3a/GSPT1 antibody (68217-1-Ig, Clone: 2A3H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HCT 116 cells, COLO 320 cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF detected in: HepG2 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68217-1-Ig targets eRF3a/GSPT1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: eRF3a/GSPT1 fusion protein Ag27986
• Full Name: G1 to S phase transition 1
• Calculated Molecular Weight: 68 aa, 4 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC009503
• Gene Symbol: GSPT1
• Gene ID (NCBI): 2935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).