eRF3a/GSPT1 Polyclonal antibody

eRF3a/GSPT1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28130-1-AP

Synonyms

551G9.2, eRF3a, ETF3A, FLJ38048, FLJ39067, G1 to S phase transition 1, GSPT1, GST1

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Validation Data Gallery

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  WB analysis of A549 using 28130-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 28130-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 28130-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 28130-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using eRF3a/GSPT1 antibody (28130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, PC-3 cells, SKOV-3 cells, Hela cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28130-1-AP targets eRF3a/GSPT1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: eRF3a/GSPT1 fusion protein Ag27963
• Full Name: G1 to S phase transition 1
• Calculated Molecular Weight: 68 aa, 4 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC009503
• Gene Symbol: GSPT1
• Gene ID (NCBI): 2935
• RRID: AB_2918149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).