GRK1 Polyclonal antibody

GRK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24606-1-AP

Synonyms

GPRK1, GRK1, Rhodopsin kinase, RHOK, RK

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Validation Data Gallery

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  WB analysis of Y79 using 24606-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 24606-1-AP (GRK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 24606-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 24606-1-AP (GRK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 24606-1-AP

  IP result of anti-GRK1 (IP:24606-1-AP, 4ug; Detection:24606-1-AP 1:500) with Y79 cells lysate 1240ug.

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  IHC staining of mouse eye using 24606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24606-1-AP (GRK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 24606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24606-1-AP (GRK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 24606-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using GRK1 antibody (24606-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Y79 cells, mouse eye tissue
• Positive IP detected in: Y79 cells
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

24606-1-AP targets GRK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRK1 fusion protein Ag18063
• Full Name: G protein-coupled receptor kinase 1
• Calculated Molecular Weight: 563 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC140945
• Gene Symbol: GRK1
• Gene ID (NCBI): 6011
• RRID: AB_2879635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRK1(G protein-coupled receptor kinase 1) is also named as RHOK, RK, GPRK1 and belongs to the AGC Ser/Thr protein kinase family. It is involved in quenching of light-induced signal transduction in phosphoreceptor. GRK1 is a photoreceptor-specific enzyme that mediates adaptation of photoreceptors to light and protects these cells against light-induced injury.

Publications

Species	Application	Title
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li
mouse	IHC	Zool Res; Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations.; Authors - Zhi-Lin Ren
zebrafish	WB	Biochim Biophys Acta Mol Basis Dis; Knockout of DLIC1 leads to retinal cone degeneration via disturbing Rab8 transport in zebrafish; Authors - Jing Zhang