GPR65 Polyclonal antibody

GPR65 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20306-1-AP

Synonyms

G protein coupled receptor 65, GPR65, hTDAG8, Psychosine receptor, TDAG8

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Validation Data Gallery

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  IHC staining of human tonsillitis using 20306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 20306-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20306-1-AP targets GPR65 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR65 fusion protein Ag14139
• Full Name: G protein-coupled receptor 65
• Calculated Molecular Weight: 337 aa, 39 kDa
• GenBank Accession Number: BC035633
• Gene Symbol: GPR65
• Gene ID (NCBI): 8477
• RRID: AB_2878668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).