GPR108 Polyclonal antibody

GPR108 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24009-1-AP

Synonyms

G protein coupled receptor 108, GPR108, LUSTR2, Protein GPR108

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Validation Data Gallery

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  WB analysis of mouse kidney using 24009-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24009-1-AP (GPR108 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 24009-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 24009-1-AP (GPR108 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24009-1-AP (GPR108 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24009-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24009-1-AP (GPR108 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 24009-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24009-1-AP (GPR108 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

24009-1-AP targets GPR108 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR108 fusion protein Ag20887
• Full Name: G protein-coupled receptor 108
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61-70 kDa
• GenBank Accession Number: BC007862
• Gene Symbol: GPR108
• Gene ID (NCBI): 56927
• RRID: AB_2879401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR108 is a seven-transmembrane family protein that falls in the GPCRs superfamily (PMID:30332431). GPR108 was identified as a highly conserved AAV entry factor implicated in cellular immune responses to AAV (PMID:31784416). Of note is its role in activating nuclear factor kappa-B (NF-κB) signaling, which implied the potential involvement of GPR108 in inflammation-related diseases as well as cancers through dysregulating NF-κB activity (PMID:35659621).

Publications

Species	Application	Title
human	WB,IF	J Virol; Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry; Authors - Timothy J Smith
human	WB	Nat Chem Biol; Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A; Authors - Xiaohui Zhao