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GPKOW Monoclonal antibody

GPKOW Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat no : 68338-1-Ig

Synonyms

G patch domain and KOW motifs, GPATC5, GPATCH5, GPKOW, Protein MOS2 homolog, Protein T54, T54

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPKOW Monoclonal antibody (68338-1-Ig)

    WB analysis using 68338-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68338-1-Ig (GPKOW antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inU2OS cells, LNcaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68338-1-Ig targets GPKOW in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPKOW fusion protein Ag6545
Full Name G patch domain and KOW motifs
Calculated Molecular Weight 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC000397
Gene Symbol GPKOW
Gene ID (NCBI) 27238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPKOW is a RNA-binding protein involved in pre-mRNA splicing.It interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosome.

Protocols

Product Specific Protocols
WB protocol for GPKOW antibody 68338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols