GPBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPBP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21622-1-AP

Synonyms

GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN

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Validation Data Gallery

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  WB analysis of HepG2 using 21622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21622-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

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  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human testis using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IHC detected in: human small intestine tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 21622-1-AP is GPBP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPBP1 fusion protein Ag14877
• Full Name: GC-rich promoter binding protein 1
• Calculated Molecular Weight: 473 aa, 53 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC000267
• Gene Symbol: GPBP1
• Gene ID (NCBI): 65056
• RRID: AB_10732822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP	Exp Mol Med; Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.; Authors - Liang-Liang Fan ; KD Validated