GPAM Polyclonal antibody

GPAM Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12454-1-AP

Synonyms

GPAM, GPAT 1, GPAT1, KIAA1560, RP11 426E5.2



Tested Applications

Positive IP detected inmouse liver tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12454-1-AP targets GPAM in IP, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPAM fusion protein Ag3120
Full Name glycerol-3-phosphate acyltransferase, mitochondrial
Calculated Molecular Weight 828 aa, 94 kDa
Observed Molecular Weight90-92 kDa
GenBank Accession NumberBC030783
Gene Symbol GPAM
Gene ID (NCBI) 57678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.

Protocols

Product Specific Protocols
IHC protocol for GPAM antibody 12454-1-APDownload protocol
IP protocol for GPAM antibody 12454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols