GNRHR Recombinant antibody

GNRHR Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7E11

Cat no : 82847-1-RR

Synonyms

GnRH R, GnRH receptor, GNRHR, GNRHR1, GRHR, LHRHR, LRHR

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Validation Data Gallery

• 

  WB analysis using 82847-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82847-1-RR (GNRHR antibody) at dilution of 1:5100 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-251 cells, MCF-7 cells, PC-3 cells, HEK-293 cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82847-1-RR targets GNRHR in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: GnRH receptor
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: ~38 kDa
• GenBank Accession Number: NM_000406
• Gene Symbol: GNRHR
• Gene ID (NCBI): 2798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.