GNAI3 Polyclonal antibody

GNAI3 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11641-1-AP

Synonyms

87U6, G(i) alpha 3, GNAI3

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Validation Data Gallery

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  WB analysis using 11641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11641-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11641-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11641-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11641-1-AP

  Immunofluorescent analysis of HepG2 cells, using GNAI3 antibody 11641-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of A431 using 11641-1-AP

  1X10^6 A431 cells were stained with .2ug GNAI3 antibody (11641-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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  FC experiment of A431 using 11641-1-AP

  1X10^6 A431 cells were surface stained with 0.2 ug Anti-Human GNAI3 (11641-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: rat kidney tissue, A431 cells, mouse brain tissue, human kidney tissue
• Positive IHC detected in: human kidney tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11641-1-AP targets GNAI3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAI3 fusion protein Ag2229
• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: ~40 kDa
• GenBank Accession Number: BC025285
• Gene Symbol: GNAI3
• Gene ID (NCBI): 2773
• RRID: AB_2111489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

Publications

Species	Application	Title
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu