GMEB2 Monoclonal antibody

• Featured Product
• KD/KO Validated

GMEB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10H8

Cat no : 68010-1-Ig

Synonyms

DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79

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Validation Data Gallery

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  WB analysis using 68010-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 68010-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68010-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of MCF-7 using 68010-1-Ig

  WB result of GMEB2 antibody (68010-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB2 transfected MCF-7 cells.

• 

  WB analysis of MCF-7 using 68010-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 68010-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, Jurkat cells, RAW 264.7 cells, HEK-293 cells, K-562 cells, PC-12 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68010-1-Ig targets GMEB2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GMEB2 fusion protein Ag31393
• Full Name: glucocorticoid modulatory element binding protein 2
• Calculated Molecular Weight: 530 aa, 56 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC036305
• Gene Symbol: GMEB2
• Gene ID (NCBI): 26205
• RRID: AB_2918757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMEB-2 (glucocorticoid modulatory element-binding protein 2), also known as PIF79 (Parvovirus initiation factor p79) or P79PIF, is a DNA-binding protein that plays a crucial role in modulating transcription upon activation by steroid hormones. GMEB-2 is ubiquitously expressed with preferential expression in developmentally important tissues.