GLT25D1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLT25D1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16768-1-AP

Synonyms

GLT25D1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 16768-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 16768-1-AP

  IP result of anti-GLT25D1 (IP:16768-1-AP, 4ug; Detection:16768-1-AP 1:500) with HeLa cells lysate 4000ug.

• 

  IHC staining of human ovary tumor using 16768-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 16768-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 16768-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 16768-1-AP is GLT25D1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLT25D1 fusion protein Ag10346
• Full Name: glycosyltransferase 25 domain containing 1
• Calculated Molecular Weight: 231 aa, 27 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC020492
• Gene Symbol: GLT25D1
• Gene ID (NCBI): 79709
• RRID: AB_2878313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Ann Neurol; Biallelic COLGALT1 variants are associated with cerebral small vessel disease.; Authors - Satoko Miyatake
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima
human	WB	Biochemistry; Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.; Authors - Masahiko Terajima ; KD Validated
mouse	WB,IHC	Mol Med Rep; Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.; Authors - Xiaohui Ye ; KO Validated