GLO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLO1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 15140-1-AP

Synonyms

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase

---

Validation Data Gallery

• 

  WB analysis using 15140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15140-1-AP (GLO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15140-1-AP

  IP result of anti-GLO1 (IP:15140-1-AP, 4ug; Detection:15140-1-AP 1:1000) with HepG2 cells lysate 1000ug.

• 

  IHC staining of human thyroid cancer using 15140-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15140-1-AP (GLO1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 15140-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15140-1-AP (GLO1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Raji cells, HeLa cells, rat liver tissue, HepG2 cells, mouse liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Product Information

15140-1-AP targets GLO1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLO1 fusion protein Ag7314
• Full Name: glyoxalase I
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC001741
• Gene Symbol: GLO1
• Gene ID (NCBI): 2739
• RRID: AB_2109890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer. The molecular mass of the dimer was 46 kDa determined by gel permeation chromatography.

Publications

Species	Application	Title
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
mouse	WB	Front Pharmacol; Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice; Authors - Jin-Bo Xie
mouse	WB,IF	Phytomedicine; Protective effects of Huang-Lian-Jie-Du Decoction on diabetic nephropathy through regulating AGEs/RAGE/Akt/Nrf2 pathway and metabolic profiling in db/db mice.; Authors - Dan Tang
human	WB	Oncotarget; Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.; Authors - Shoma Tamori ; KD Validated
mouse	WB	Life Sci Alliance; Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.; Authors - Shengya Tian
rat	WB	Free Radic Biol Med; Methylglyoxal accumulation contributes to accelerated brain aging in spontaneously hypertensive rats; Authors - Wen-Yuan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Billie (Verified Customer) (07-10-2021)

Strong band at around 25 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 with 10ug protein loaded
• Cell Tissue Type: Astrocytes