GLI4 Polyclonal antibody

GLI4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 16551-1-AP

Synonyms

GLI family zinc finger 4, GLI4, HKR4, Protein HKR4, Zinc finger protein GLI4

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Validation Data Gallery

• 

  WB analysis of Jurkat using 16551-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16551-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16551-1-AP targets GLI4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLI4 fusion protein Ag9497
• Full Name: GLI family zinc finger 4
• Calculated Molecular Weight: 376 aa, 41 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC014165
• Gene Symbol: GLI4
• Gene ID (NCBI): 2738
• RRID: AB_2109716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

iru (Verified Customer) (12-14-2020)

It works at 1:500 O/N incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fallopian tube epithelial cells