GK3P Polyclonal antibody

GK3P Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14176-1-AP

Synonyms

GK3P, GKP3, GKTB, glycerol kinase 3 pseudogene

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Validation Data Gallery

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  WB analysis of HeLa using 14176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14176-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 14176-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin cancer using 14176-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14176-1-AP targets GK3P in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GK3P fusion protein Ag5383
• Full Name: glycerol kinase 3 pseudogene
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC066960
• Gene Symbol: GK3P
• Gene ID (NCBI): 2713
• RRID: AB_1288669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.