GIMAP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

GIMAP7 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17293-1-AP

Synonyms

GIMAP7, GTPase IMAP family member 7, GTPase, IMAP family member 7, hIAN7, IAN 7, IAN7

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 17293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17293-1-AP (GIMAP7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 17293-1-AP

  IP result of anti-GIMAP7 (IP:17293-1-AP, 4ug; Detection:17293-1-AP 1:800) with Jurkat cells lysate 1080ug.

• 

  IF Staining of HepG2 using 17293-1-AP

  Immunofluorescent analysis of HepG2 cells, using GIMAP7 antibody 17293-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

17293-1-AP targets GIMAP7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIMAP7 fusion protein Ag11171
• Full Name: GTPase, IMAP family member 7
• Calculated Molecular Weight: 300 aa, 35 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC027613
• Gene Symbol: GIMAP7
• Gene ID (NCBI): 168537
• RRID: AB_2111885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIMAP7 is also name as hIAN7 or IAN 7. The function of GIMAP7 remains largely unknown. Catalog#17293-1-AP is a rabbit polyclonal antibody raised against the full-length of human GIMAP7. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

Publications

Species	Application	Title
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human,rat	WB,IHC	J Ovarian Res; GIMAP7 induces oxidative stress and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inhibiting sonic hedgehog signalling pathway; Authors - Anran Xu ; KD Validated
human,mouse	WB,IHC	Thorac Cancer; GIMAP7 inhibits epithelial-mesenchymal transition and glycolysis in lung adenocarcinoma cells via regulating the Smo/AMPK signaling pathway; Authors - Liyuan Cui ; KD Validated