GGPS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GGPS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29707-1-AP

Synonyms

GGPP synthase, GGPPS, GGPPS1, GGPPSase, GGPS1

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Validation Data Gallery

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  WB analysis using 29707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29707-1-AP (GGPS1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29707-1-AP

  WB result of GGPS1 antibody (29707-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGPS1 transfected HEK-293 cells.

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  WB analysis using 29707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29707-1-AP (GGPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 29707-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29707-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29707-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29707-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 29707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29707-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 29707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29707-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, HEK-293 cells, mouse brain tissue, mouse heart tissue
• Positive IHC detected in: mouse testis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29707-1-AP targets GGPS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GGPS1 fusion protein Ag31476
• Full Name: geranylgeranyl diphosphate synthase 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC067768
• Gene Symbol: GGPS1
• Gene ID (NCBI): 9453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.