GEMIN8 Monoclonal antibody

GEMIN8 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A6B4

Cat no : 67871-1-Ig

Synonyms

FAM51A1, FLJ20514, FLJ43072, Gem associated protein 8, Gemin 8, GEMIN8, Protein FAM51A1

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Validation Data Gallery

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  WB analysis of HeLa using 67871-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67871-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67871-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GEMIN8 antibody (67871-1-Ig, Clone: 1A6B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67871-1-Ig targets GEMIN8 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GEMIN8 fusion protein Ag6774
• Full Name: gem (nuclear organelle) associated protein 8
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC003607
• Gene Symbol: GEMIN8
• Gene ID (NCBI): 54960
• RRID: AB_2918629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.