GDAP1 Monoclonal antibody

GDAP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E10D4

Cat no : 68083-1-Ig

Synonyms

CMTRIA, GDAP1

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Validation Data Gallery

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  WB analysis using 68083-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68083-1-Ig (GDAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  IHC staining of rat brain using 68083-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 68083-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 68083-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 68083-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, Rabbit brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68083-1-Ig targets GDAP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GDAP1 fusion protein Ag28616
• Full Name: ganglioside-induced differentiation-associated protein 1
• Calculated Molecular Weight: 358 aa, 41 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC024939
• Gene Symbol: GDAP1
• Gene ID (NCBI): 54332
• RRID: AB_2918820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.