GBA Polyclonal antibody

GBA Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20622-1-AP

Synonyms

GBA, GBAP

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Validation Data Gallery

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  IHC staining of human breast cancer using 20622-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20622-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 20622-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20622-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 20622-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20622-1-AP (GBA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20622-1-AP is GBA Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBA fusion protein Ag13981
• Full Name: glucosidase, beta; acid, pseudogene
• Calculated Molecular Weight: 321 aa, 36 kDa
• GenBank Accession Number: BC000349
• Gene Symbol: GBAP
• Gene ID (NCBI): 2630
• RRID: AB_10696317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.