GATM Polyclonal antibody

GATM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12801-1-AP

Synonyms

AGAT, AT, GATM, Transamidinase



Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, rat kidney tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12801-1-AP targets GATM in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATM fusion protein Ag3754
Full Name glycine amidinotransferase (L-arginine:glycine amidinotransferase)
Calculated Molecular Weight 423 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC004141
Gene Symbol GATM
Gene ID (NCBI) 2628
RRIDAB_2109060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.

Protocols

Product Specific Protocols
WB protocol for GATM antibody 12801-1-APDownload protocol
IHC protocol for GATM antibody 12801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Biomed Pharmacother

Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia.

Authors - Qiong Lai
mouseWB

J Agric Food Chem

Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.

Authors - Sho Nishikawa
mouseWB

Front Physiol

Arginine:Glycine Amidinotransferase Is Essential for Creatine Supply in Mice During Chronic Hypoxia.

Authors - Juliane Hannemann
mouseWB

Front Nutr

Influence of homoarginine on creatine accumulation and biosynthesis in the mouse

Authors - Craig A. Lygate
mouseWB

Amino Acids

Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin exposure.

Authors - Ali Sasani
humanIF

Gene

Clinical and functional characterization of rare compound heterozygous mutations in the SERPINC1 gene causing severe thrombophilia

Authors - Ke Zhang