CoraLite® Plus 488-conjugated GALE Monoclonal antibody
GALE Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3F2C2
Cat no : CL488-67772
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67772 targets GALE in FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | GALE fusion protein Ag6571 |
Full Name | UDP-galactose-4-epimerase |
Calculated Molecular Weight | 38 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC050685 |
Gene Symbol | GALE |
Gene ID (NCBI) | 2582 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 GALE antibody CL488-67772 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |