CoraLite® Plus 488-conjugated GAD1 Monoclonal antibody

GAD1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D5B7

Cat no : CL488-67648

Synonyms

FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-67648

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 GAD1 antibody (CL488-67648, Clone: 2D5B7 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-67648

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 GAD1 antibody (CL488-67648, Clone: 2D5B7 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67648 targets GAD1 in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAD1 fusion protein Ag21336
• Full Name: glutamate decarboxylase 1 (brain, 67kDa)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC002815
• Gene Symbol: GAD1
• Gene ID (NCBI): 2571
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development(PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.