GAD1 Monoclonal antibody, PBS Only

GAD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5B7

Cat no : 67648-1-PBS

Synonyms

FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP

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Validation Data Gallery

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  WB analysis of pig brain using 67648-1-Ig (same clone as 67648-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67648-1-PBS targets GAD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAD1 fusion protein Ag21336
• Full Name: glutamate decarboxylase 1 (brain, 67kDa)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa, 57 kDa
• GenBank Accession Number: BC002815
• Gene Symbol: GAD1
• Gene ID (NCBI): 2571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.